A stereospecific solid-phase screening assay for colonies expressing both ( R )- and ( S )-selective ω-aminotransferases

Stereospecific Assay of (R)- and (S)-Goitrin in Commercial Formulation of Radix Isatidis by Reversed Phase High-Performance Liquid Chromatography

Radix isatidis (Banlangen) is a widely used traditional Chinese medicine for treating fever and removing toxic heat. Pharmacological studies have indicated that (R)-goitrin (epigoitrin) is one of the main constituents accounting for its antiviral activity, while (S)-goitrin (goitrin) is known as an antithyroid factor. To better control the quality of radix isatidis and its formulations, it is i...

The Electrochemical Sensor for Selective Solid Phase Extraction of Pseudoephedrine Hydrochloride in a Real Sample

In  the  current  study,  a  new  technique  was  developed  for  quantification  and  qualification  of pseudoephedrine hydrochloride (PSE) in a real sample, which was based on electrochemical sensors and  molecular  imprinted  polymer  (MIP).  The  carbon  paste  electrode  (CPE)  was  modified  and optimized by a different ratio of MIP. MIP/CPE was used as the extraction and working electrod...

Screening Assay for Selective Estrogen Receptor Modulators

A Solid-Phase Enzyme-Linked Assay for Vitamin B12

A new solid-phase enzyme-linked competitive binding assay for vitamin B12 (cyanocobalamin) is described. The assay is based on the competition between analyte B12 molecules and a glucose-6-phosphate dehydrogenase-vitamin B12 conjugate for a limited number of R-protein binding sites immobilized on sepharose particles. After appropriate incubation and washing steps, the enzyme activity bound to t...

A practical algorithm for [r, s, t]-coloring of graph

Coloring graphs is one of important and frequently used topics in diverse sciences. In the majority of the articles, it is intended to find a proper bound for vertex coloring, edge coloring or total coloring in the graph. Although it is important to find a proper algorithm for graph coloring, it is hard and time-consuming too. In this paper, a new algorithm for vertex coloring, edge coloring an...